Quantification and characterization of putative diazotrophic bacteria from forage palm under saline water irrigation

Gabiane dos Reis Antunes, Thaise Rosa da Silva, Beatriz Rodrigues Carvalho, José Neuman Miranda Neiva, Gherman Garcia Leal de Araújo, Paulo Ivan Fernandes Júnior


The aim of this study was to evaluate the density and phenotypical diversity of diazotrophic endophytic bacteria from the forage palm irrigated with different saline water depths. Opuntia stricta (IPA-200016) received five depths of saline water (L1: 80%. ETo; L2: 60%.ETo; L3: 40%; ETo; L4: 20%; ETo and, L5: 0% ETo, where ETo is the reference evapotranspiration). The roots were collected in the field, disinfected, grounded and serial diluted from 10-1 to 10-4. The total concentration of diazotrophic bacteria was determined by the most probable number method (MPN) and the isolated bacteria were characterized phenotipically. The concentration of bacteria found in forage palm roots ranged from 0.36 x 104 to 109.89 104 cells per gram of root, with highest occurrence on the 60 and 80% ETo. In the dendrogram of similarity it was possible to observe the formation of 24 phenotypic groups with 100% similarity. All bacteria presented similarity superior to 40%. Among these groups, 14 are rare groups, formed by only a single bacterial isolate. In the Semi-Arid conditions, the forage palm that receives the highest amount of saline water, presents a higher density of putative nitrogen-fixing endophytic bacteria with high phenotypic diversity.


Microorganisms, Forage, Biossaline Agriculture, Inoculant

Texto completo:



ARAUJO, G.G.L., VOLTOLINI, T.V., CHIZZOTTI, M.L., TURCO, S.H.N., CARVALHO, F.F.R. Water and small ruminant production. Brazilian Journal of Animal Science, Piracicaba, v. 39, 326-336, 2010.

BACON, C.W., HINTON, D.M. AND HINTON JR, A. Growth-inhibiting effects of concentrations of fusaric acid on the growth of Bacillus mojavensis and other biocontrol Bacillus species. Journal of Applied Microbiology v. 100, n.1, p.185-194, 2006

BALDANI, J.I.; AZEVEDO, M.S.; REIS, V.M.; TEIXEIRA, K.R.S.; OLIVARES, F.L.; GOI, S.R.; BALDANI, V.L.D. & DÖBEREINER, J. Fixação biológica de nitrogênio em gramíneas: avanços e aplicações. In: SIQUEIRA, J.O.; MOREIRA, F.M.S.; LOPES, A.S.; GUILHERME, L.R.G.; FAQUIN, V.; FURTINI NETO, A.E. & CARVALHO, J.G., eds. Inter-relação fertilidade, biologia do solo e nutrição de plantas. Viçosa, SBCS/UFLA/DCS p.621-666, 1999.

BALDANI, J.I.; REIS, V.M.; VIDEIRA, S.S.; BODDEY, L.H.; BALDANI, V.L.D. The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant and Soil, v.384, p.413-431, 2014.

COMPANT, S.; CLEMENT, C.; SESSITSCH, A. Plant growth-promoting bacteria in the rhizo-and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry, Oxford, v.42, p.669-678, 2010.

DOBEREINER, J.; BALDANI, V.L.D.; BALDANI, J.I. Como isolar e identificar bactérias diazotróficas de plantas não leguminosas. Brasília: Embrapa, SPI, 60p., 1995.

ESPAÑA-GAMBOA, E.; MIJANGOS-CORTES, J.; BARAHONA-PEREZ, L.; DOMINGUEZ-MALDONADO, J.; HERNÁNDEZ-ZARATE, G.; ALZATEGAVIRIA, L. Vinasses: characterization and treatments. Waste Management & Research, v.29, p.1235-1250, 2011.

ESTRADA DE LOS SANTOS, P.; BUSTILLOS-CRISTALES, R.; ABALLEROMELLADO, J. Burkholderia a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Applied and Environmental Microbiology, v.67, p.2790-2798, 2001.

FLOWERS, T.J.; COLMER, T.D. Salinity tolerance in halophytes. New Phytol. v. 179, p.945-950, 2008.

GLICK, B.R. Plant growth-promoting bacteria: mechanisms and applications. Hindawi Publishing Corporation, Scientifica, 2012.

GROVER, M.; ALI, S.Z.; SANDHYA, V.; RASUL, A.; VENKATESWARLU, B. Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World Journal of Microbiology and Biotechnology v. 27, n.5, p.1231-1240, 2011.

HAMMER, O.; HARPER, D. A. T.; RYAN, P. D. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, Boulder, v. 4, n. 1, p. 1-9, 2001.

HUNGRIA, M.; ARAUJO, R. Manual de métodos empregados em estudos de microbiologia agrícola. Brasília, Empresa Brasileira de Pesquisa Agropecuária, 542p., 1994.

JOHRI, B.N. Endophytes to the rescue of plants. Current Science v. 90, n.10, p.1315-1316, 2006.

LIMA, G. F. C.; RÊGO, M. M. T.; AGUIAR, E. M. l.; SILVA, J.G.M.; DANTAS, F.D.G.; GUEDES, F.X.; LÔBO, R.N.B. Effect of different cutting intensities on morphological characteristics and productivity of irrigated Nopalea forage cactus. In: International Congress on Cactus Pear and Cochineal, 8., 2013. Palermo: ISHS Acta Horticulturae, v. 1067. p. 253- 258, 2015.

LIMA, J.V.L.; WEBER, O.B.L.; CORREIA, D.; SOARES, M.A.; SENABIO, J.A. Endophytic bacteria in cacti native to a Brazilian semi-arid region. Plant and Soil n.389: p.25–33, 2015.

LOPES, E. L. N. et al. Microbial biomass and soil chemical properties under different land use systems in northeastern Pará. Revista. Brasileira de Ciência do Solo, v. 35, n. 4, p. 1127-1139, 2011.

MANOUSAKI, E.; KALOGERAKIS, N. Halophytes present new opportunities in phytoremediation of heavy metals and saline soils. Industrial e Engineering Chemistry Research, v. 50, p.656-660, 2011.

MISHRA, R. R.; PRAJAPATI, S.; DAS, J.; DANGAR, T. K.; DAS, N.; THATOI, H. Reduction of selenite to red elemental selenium by moderately halotolerant Bacillus megaterium strains isolated from Bhitarkanika mangrove soil and characterization of reduced product. Chemosphere, v.84, p.1231-1237, 2011.

MOREIRA, F. M. S.; SIQUEIRA, J. O. Microbiologia e Bioquímica do Solo. 2 ed. Lavras, UFLA, 2006, 729 p.

MOREIRA, F. T. A., SANTOS, D. R., SILVA, G. H., ALENCAR, L. S. Ocorrência De Bactérias do Gênero Azospirillum spp. associadas a gramíneas forrageiras no Semiárido Nordestino. HOLOS, Ano 29, Vol. 3. 2013.

OSAKI, F.; NETTO, S. P. Flutuação da população de bactérias sob floresta ombrófila mista e povoamento de Pinus taeda L. Floresta, v. 39, p. 845-852, 2009.

REIS, V.M.; TEIXEIRA, K.R.S. Fixação biológica do nitrogênio - Estado da arte. In: Aquino AM, Assis RL, editores. Processos biológicos no sistema solo-planta: ferramentas para uma agricultura sustentável. Brasília: Embrapa Informação Tecnológica, p.350-68, 2005.

RODRIGUES NETO, J.; MALAVOLTA, J.R.; VICTOR, O. Meio simples para isolamento e cultivo de Xanthomonas campestris pv. citri tipo B. Suma Phytopathologica, v.12, p.16, 1986.

SANTOS, M.C.M.; SANTOS, D.R.; BAKKE, O.A.; BAKKE, I.A. Ocorrência e Atividade de Bactérias Diazotróficas em Forrageiras cultivadas na região Semiárida no Brasil. Revista Caatinga, Mossoró, v. 26, n. 1, p. 27-34, 2013.

QADIR, M.; OSTER, J. D.; SCHUBERT, S.; NOBLE, A. D.; SAHRAWAT, K. L. Phytoremediation of sodic and saline-sodic soils. Advances in Agronomy, v.96, p.197-247, 2007.


  • Não há apontamentos.

Licença Creative Commons