Anatomical characterization, histochemistry and crystal analysis of the leaf blade of Mangifera indica L.

Rafaela Damasceno Sá, Girllene da Silva Cavalcanti, Deysielle Maria dos Santos, Adolfo Santos da Silva, Rafael José Ribeiro Padilha, Luiz Carlos Alves, Karina Perrelli Randau

Resumo


Mangifera indica L., popularly known as mangueira, is an important fruitful widely used in Brazilian traditional medicine for the treatment of diabetes, anemia, diarrhea, hemorrhoids, indigestion, asthma, bronchitis, flu, hypertension, rheumatism, disorders of the liver and for tumors. In order to expand the pharmacobotanical information about the species, this study aimed to perform the anatomical characterization, histochemistry and crystal analysis of the leaf blade of M. indica. For the anatomical characterization were used optical microscopy, polarization microscopy and scanning electron microscopy. Histochemical tests were performed to evidence the presence of metabolites and microchemical analyzes by dispersive energy spectroscopy were done to determine the elemental chemical composition of the crystals. The microscopic analysis allowed the identification of characters useful in the identification of the species, such as the presence of glandular trichomes in the leaf blade. Through histochemistry was evidenced the presence of phenolic compounds, lipophilic substances, lignin and starch in the leaf blade. The chemical nature of the crystals was determined to be calcium oxalate. The results contribute to the pharmacobotanical standardization of M. indica.

Palavras-chave


Anacardiaceae; Mangueira; Quality control

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Referências


BUKATSCH, F. 1972. Bemerkungen zur doppelfärbung Astrablau-Safranin. Mikrokosmos, v. 61, p. 255.

DHITAL, K. S. 2017. Phytochemical screening and antioxidant activities of Mangifera indica leaves grown in temperate region of the Nepal. J. Pharmacogn. Phytochem., v. 6, n. 3, p. 205-209.

DISO, S. U.; ALI, M.; MUKHTAR, S. I.; GARBA, M. 2017. Antibacterial activity and phytochemical screening of Mangifera indica (mango) stem and leaf extracts on clinical isolates of methicillin resistant Staphylococcus aureus. J. Adv. Med. Pharm. Res., v. 13, n. 1, p. 1-6.

DIVYALASHMI, L.; SHARMILI, A. 2017. Phytochemical analysis and antibacterial activity of Mangifera indica L and Piper betle. Int. J. Pharm. Bio. Sci., v. 8, n. 2, p. 84-91.

EDIRIWEERA, M. K.; TENNEKOON, K. H.; SAMARAKOON, S. R. 2017. A review on ethnopharmacological applications, pharmacological activities, and bioactive compounds of Mangifera indica (mango). Evid. Based Complementary Altern. Med., v. 2017.

FRANCESCHI, V. R.; NAKATA, P. A. 2005. Calcium oxalate in plants: formation and function. Annu. Rev. Plant Biol., v. 56, n. 1, p. 41-71.

GABE, M. 1968. Techniques histologiques. Paris: Masson & Cie.

GHUNIYAL, J. 2015. Ethanomedical, chemical, pharmacological, toxicological properties of Mangifera indica: a review. Int. J. Pharm. Res. Rev., v. 4, n. 10, p. 51-64.

HADDAD, A.; SESSO, A.; ATTIAS, M.; FARINA, M.; MEIRELLES, M. N.; SILVEIRA, M.; BENCHIMOL, M.; SOARES, M. J.; BARTH, O. M.; MACHADO, R. D.; SOUTO-PADRÓN, T.; SOUZA, W. 1998. Técnicas básicas de microscopia eletrônica aplicadas às Ciências Biológicas. Rio de Janeiro: Sociedade Brasileira de Microscopia Eletrônica.

HELEN, P. A. M.; ASWATHY, M. R.; DEEPTHI, K. G.; MOL, R. R.; JOSEPH, J. J.; SREE, S. J. 2013. Phytochemical analysis and anticancer activity of leaf extract of Mangifera indica (kottukonam varika). Int. J. Pharm. Sci. Res., v. 4, n. 2, p. 823-828.

ILARSLAN, H.; PALMER, R. G.; IMSANDE, J.; HORNER, H. T. 1997. Quantitative determination of calcium oxalate and oxalate in developing seeds of soybean (Leguminosae). Amer. J. Bot., v. 84, p. 10422-1046.

JAHURUL, M. H. A.; ZAIDUL, I. S.; GHAFOOR, K.; AL-JUHAIMI, F. Y.; NYAM, K. L.; NORULAINI, N. A.; SAHENA, F.; MOHD OMAR, A. K. 2015. Mango (Mangifera indica L.) by-products and their valuable components: A review. Food Chem., v. 183, p. 173-180.

JENSEN, W. A. 1962. Botanical histochemistry, principles and practice. San Francisco: W. H. Freeman.

JOHANSEN, D. A. 1940. Plant microtechnique. New York: McGraw-Hill Book Co. Inc.

KRAUS, J. E.; ARDUIN, M. 1997. Manual básico em métodos de morfologia vegetal. Rio de Janeiro: EDUR.

KRAUTER, D. 1985. Erfahrungen mit Etzolds FSA-Färbung für pflanzenschnitte. Mikrokosmos, v. 74, p. 231-233.

LACCHIA, A. P. S.; CARMELLO-GUERREIRO, S. M. 2009. Aspectos ultra estruturais dos canais secretores em órgãos vegetativos e reprodutivos de Anacardiaceae. Acta Bot. Brasilica, v. 23, n. 2, p. 376-378.

LAURICELLA, M.; EMANUELE, S.; CALVARUSO, G.; GIULIANO, M.; D'ANNEO, A. 2017. Multifaceted health benefits of Mangifera indica L. (mango): the inestimable value of orchards recently planted in sicilian rural areas. Nutrients v. 9, n. 5, p. E525.

LORENZI, H.; LACERDA, M. T. C.; BACHER, L. B. 2015. Frutas no Brasil: nativas e exóticas (de consumo in natura). São Paulo: Instituto Plantarum de Estudos da Flora.

MACE, M. E.; BELL, A. A.; STIPANOVIC, R. D. 1974. Histochemistry and isolation of gossypol and related terpenoids in root of cotton seedlings. Phytopathology v. 64, p. 1297-1302.

MACE, M. Z.; HOWELL, C. R. 1974. Histochemistry and identification of condensed tannin precursors in roots of cotton seedlings. Can. J. Bot. v. 52, n. 11, p. 2423-2426.

METCALFE, C. R.; CHALK, K. L. 1950. Anatomy of the dicotyledons: leaves, stem, and wood in relation to taxonomy with notes on economic uses. Oxford: Clarendon.

NWANKWO, I. U.; OSARO-MATHEW, R. C. 2014. Assessment of the phytochemical components of Mangifera indica (leaf) and Musa paradisiaca (roots) extracts and their antibacterial activity against some common pathogenic bacteria. J. Pharm. Biol. Sci., v. 9, n. 1, p. 8-11.

OKWU, D. E.; EZENAGU, V. 2008. Evaluation of the phytochemical composition of mango (Mangifera indica Linn) stem bark and leaves. Int. J. Chem. Sci., v. 6, n. 2, p. 705-716.

OLIVEIRA, F.; AKISUE, G. 2009. Fundamentos de farmacobotânica e de morfologia vegetal. 3ª ed. São Paulo: Atheneu.

PARVEZ, G. M. M. 2016. Pharmacological activities of mango (Mangifera indica): a review. J. Pharmacogn. Phytochem., v. 5, n. 3, p. 1-7.

PELL, S. K.; MITCHELL, J. D.; MILLER, A. J.; LOBOVA, T. A. Anacardiaceae. In: KUBITZKI, K. 2011. Eudicots: Sapindales, Cucurbitales, Myrtaceae. India: Springer-Verlag Berlin Heidelberg, p. 7-50.

RIBEIRO, R. V.; BIESKI, I. G. C.; BALOGUN, S. O.; MARTINS, D. T. O. 2017. Ethnobotanical study of medicinal plants used by Ribeirinhos in the North Araguaia microregion, Mato Grosso, Brazil. J. Ethnopharmacol., v. 205, p. 69-102.

ROCHA, L. A.; ROCHA, A. M.; PACHECO, A. C. L.; ABREU, M. C. 2015. Diferenças foliares morfoanatômicas de quatro espécies da família Anacardiaceae. Caderno de Pesquisa, série Biologia, v. 27, n. 2, p. 35-48.

SANTHAN, P. 2014. Leaf structural characteristics of importante medicinal plants. Int. J. Res. Ayurveda Pharm., v. 5, n. 6, p. 673-679.

SANTOS, M. M.; NUNES, M. G. S.; MARTINS, R. D. 2012. Uso empírico de plantas medicinais para tratamento de diabetes. Rev. Bras. Pl. Med., v. 14, n. 2, p. 327-334.

SASS, J. E. 1951. Botanical microtechnique. 2nd. ed. Ames: The Iowa State College Press.

SHAH, K. A.; PATEL, M. B.; PATEL, R. J.; PARMAR, P. K. 2010. Mangifera indica (Mango). Pharmacogn Rev., v. 4, n. 7, p. 42-48.

SOMKUWAR, D. O.; KAMBLE, V. A. 2013. Phytochemical screening of ethanolic extracts of stem, leaves, flower and seed kernel of Mangifera indica L. Int. J. Pharm. Bio. Sci., v. 4, n. 2, p. 383-389.

VASCONCELOS, A. L.; VASCONCELOS, A. L.; RANDAU, K. P. 2016. Pharmacognostic characterization of Spondias mombin L. (Anacardiaceae). Pharmacogn. J., v. 8, n. 6, p. 513-519.

YODER, L. R.; MAHLBERG, P. G. 1976. Reactions of alkaloid and histochemical indicators in laticifers and specialized parenchyma cells of Catharanthus roseus (Apocynaceae). Am. J. Bot., v. 63, n. 9, p. 1167-1173.




DOI: https://doi.org/10.24221/jeap.4.4.2019.2631.266-272

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Direitos autorais 2020 Rafaela Damasceno Sá, Girllene da Silva Cavalcanti, Deysielle Maria dos Santos, Adolfo Santos da Silva, Rafael José Ribeiro Padilha, Luiz Carlos Alves, Karina Perrelli Randau

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Esta obra está licenciada sob uma licença Creative Commons Atribuição - Não comercial - Compartilhar igual 4.0 Internacional.

Obra licenciada com uma Licença Creative Commons Atribuição-NãoComercial-CompartilhaIgual 4.0 Internacional.